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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but specific nucleotide sequence info is not nevertheless accessible.

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Blocking peptides are peptides that bind exclusively to the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.

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This guideline outlines further more doable optimization of cellular and immunoassay parameters to make sure the best possible success are...

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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...

Antibodies certain to the blocking peptide now not bind for the epitope to the focus on protein. By evaluating the staining with the blocked antibody versus the antibody on your own, one can see which staining is specific.

Target Information and facts Cyclin E belongs into the really conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation styles which contribute for the temporal coordination of each and every mitotic party. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells development by S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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